FastQCFastQC Report
Wed 8 May 2024
EGAF00007947322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834136
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG104008413.276307687280383Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950271.2129863459097467No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAG190980.2437792757235769TruSeq Adapter, Index 11 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACT160560.20494921201265845TruSeq Adapter, Index 11 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC130470.16654038173450142Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)