FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8820465
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG6429557.289354926299237Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA681200.7722948846801161No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGA211060.23928443681824033TruSeq Adapter, Index 25 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGT170820.19366325924993752TruSeq Adapter, Index 25 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAA146700.16631776215879776TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTA124450.14109233470117505TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAA115650.13111553642580068TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACT112260.12727220163562805TruSeq Adapter, Index 25 (97% over 40bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTA111420.1263198708911605TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAT107070.12138815810731067TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTC104570.11855384041544294TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAA102050.1156968481820403TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTT101610.11519800826827156TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAT95710.10850901851546377TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAC94940.1076360486663685TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAC93930.10649098431885394TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACA93390.10587877169741051TruSeq Adapter, Index 25 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)