FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5385386
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG69911312.98166928053068Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT331020.6146634614491886TruSeq Adapter, Index 3 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA299880.5568403081970354TruSeq Adapter, Index 3 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224760.41735169958105145No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTC223560.4151234470472497TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAA171950.31929001932266327TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTG169470.3146849640861398TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC164530.30551199115532296TruSeq Adapter, Index 3 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTA153580.2851791867843828TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG134480.2497128339547063TruSeq Adapter, Index 3 (96% over 33bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA134030.24887723925453067No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGC129060.2396485600103688TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGT126410.23472783566489014TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTT122060.2266504202298591TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCA119140.22122833906427505TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA118590.2202070566529493No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCT110340.20488782048306287TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT103770.19268813786049876Illumina PCR Primer Index 2 (96% over 31bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT103130.19149973650913787TruSeq Adapter, Index 1 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA100930.18741460686383485No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGT100590.18678326864592437TruSeq Adapter, Index 1 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG98730.1833294772185318TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGA95120.1766261508460118TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTC93860.17428648568552002TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAT91940.17072128163143738TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCT91280.16949574273784646TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA91050.16906866100220114TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTT90750.16851159786875072TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTA90420.16789882842195525TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAG90380.1678245533374952TruSeq Adapter, Index 1 (97% over 36bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC89430.1660605200815689No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAT87250.16201252797849586TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGC86980.1615111711583905TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAC85920.15954288142019904TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAA85010.15785312324873277TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAC83610.15525349529263083TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG82230.15269100487875892TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGA78780.14628477884407914TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAT78190.14518922134829335TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTG77590.1440750950813925TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAA76700.14242247445215628TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCT76250.14158687975198064TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAC76230.1415497422097506TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTC75280.13978570895382428TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTA74960.13919150827814386TruSeq Adapter, Index 1 (97% over 36bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG74920.1391172331936838No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAA74720.1387458577713835TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAT74320.13800310692678297TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGG74240.13785455675786285TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG72410.1344564716438153TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC72300.13425221516155017TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTG70140.13024136060070718TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTT70090.13014851674513211TruSeq Adapter, Index 1 (97% over 36bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC69550.12914580310492135No Hit
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC69420.12890440908042616RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGACT69290.128663015055931TruSeq Adapter, Index 1 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC67850.125989112015369Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCA66410.12331520897480701TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCG66320.12314809003477188TruSeq Adapter, Index 1 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCT66140.12281385215470163TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCA65470.12156974448999572TruSeq Adapter, Index 1 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT64200.11921151055838894Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTT64010.11885870390720368TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCC63360.11765173378472779TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGG63200.11735463344688754TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAG62760.11653760751782695TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTA62420.11590626929991647TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGACA60830.11295383469262929TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAA60270.11191398351018848TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGGC59870.11117123266558794TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAC58860.10929578678297155TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTC58770.10912866784293643TruSeq Adapter, Index 1 (97% over 36bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC58530.1086830173361761No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG56320.10457931891975802No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCGT56240.10443076875083791TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAT55810.10363231159289232TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAGT55210.1025181853259915TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCC54760.10168259062581586TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCGC54560.1013112152035156TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCCT54220.10067987698560513TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)