FastQCFastQC Report
Fri 10 May 2024
EGAF00007947333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5385386
Total Bases813.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG69899012.979385321683534Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG952151.768025541716044No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC147810.2744650058510198No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGT138570.25730746134074695Illumina Single End Sequencing Primer (100% over 33bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC133230.24739173756532956No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA111190.20646616602783904No Hit
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA104370.1938022641273996Illumina Single End PCR Primer 1 (100% over 25bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA90650.1683259101576006No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT90510.16806594736199038Illumina Single End PCR Primer 1 (96% over 26bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA89670.16650617058832923No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89380.16596767622599382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77770.14440933296146274No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA74980.1392286458203739Illumina Single End PCR Primer 1 (100% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA71620.13298953872572922Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG67440.12522779239965343Illumina Single End PCR Primer 1 (100% over 32bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC66340.12318522757700191No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT65660.12192255114118096Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTG62310.11570201281765131Illumina Single End Sequencing Primer (100% over 33bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGTAGATC60100.11159831440123327Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT59510.11050275690544745No Hit
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG58050.10779171632265543No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGTAGATC57520.10680757145355968Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)