FastQCFastQC Report
Wed 8 May 2024
EGAF00007947340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612490
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG307530631.99281351658103Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTAT1268011.319127510145654TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATG544020.5659511739414034TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG517880.5387573875239402TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC419170.4360680739329768Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGC409980.4265075958466537TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCA396220.41219288654656594TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAA311580.32414077934021257TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCT303690.31593270838253146TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCC303090.3153085204770044TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAC295970.3079014906647497TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAA291060.30279355297118643TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT282710.29410693795260123Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGC279480.29074672639451377TruSeq Adapter, Index 3 (96% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGA271660.2826114773591442TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAT250050.26013030962841055TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAC250020.26009910023313415TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTA247550.25752952668871437TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGT243880.2537115773332404TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCA243360.2531706144817836TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTA241200.2509235380218861TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAT240950.25066345972791654TruSeq Adapter, Index 6 (97% over 36bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGT239290.24893653985595823TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGACA237120.24667906026430197TruSeq Adapter, Index 6 (97% over 36bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC236770.2463149506527445Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTC233480.24289232030410435TruSeq Adapter, Index 6 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT226910.2360574627385828Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGA222300.23126161899778308TruSeq Adapter, Index 3 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAAC215880.2245828084086433TruSeq Adapter, Index 6 (97% over 36bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC214760.22341765765165944TruSeq Adapter, Index 5 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGACC209220.21765432265729276TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTT200700.20879085439880823TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCC198110.20609644327328297TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGACT195420.20329800083016988TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTCA194710.20255937847529618TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAAA193390.20118616508313664TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCG191270.19898070115027428TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATC187980.1955580708016341TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTT187420.1949754954231422TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTAA185790.19327978494646028TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTAC185470.19294688473017918TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTC183990.19140722122987905TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCT174650.18169069616717418TruSeq Adapter, Index 6 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTA172840.17980772931883413TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGT172230.1791731382815483TruSeq Adapter, Index 3 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAAT171860.17878822240647324TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTCT163560.17015362304668197TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTTC157620.1639741627819639TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTAT156800.16312110597774354TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATA156720.16303788092367325TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTTA150990.15707688642588966TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTCC147590.15353982162790286TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGC146800.15271797421895886TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAG145540.151407179617352TruSeq Adapter, Index 6 (97% over 36bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC140390.1460495667615779TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCGA139430.14505086611273457TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATT138940.14454111265655412TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATG132320.13765423943223867TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT130950.13622901038128518Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTTT129810.13504305336078373TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCGC129040.1342420122153573TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTG127650.13279597690088626TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG124920.12995592193073802Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT123170.1281353738729507Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAG121370.12626281015636948TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCGT116990.12170623844602178TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG113730.11831481749265799Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTG108840.11322768606261228TruSeq Adapter, Index 6 (97% over 36bp)
CAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT108200.11256188563005007Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGG108100.11245785431246223TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGCC107540.11187527893397027TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGCA104990.10922248033548018TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCG102370.10649685981467862TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAGC101960.10607033141256843TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGA101660.10575823745980491TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGGCTGAACTCCAG101330.105414934111765Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGG100690.10474913367920279TruSeq Adapter, Index 3 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)