FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612490
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG279473229.073965226491783Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1699411.7679186142196248No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGT785050.8166978587233901Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGT530210.5515844489825217Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG437830.4554803177948689Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTG383440.3988976841588392Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT357070.371464625710924Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG354060.3683332830515298Illumina Single End PCR Primer 1 (100% over 32bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGA278790.2900289103031577Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG276300.2874385304952203Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAT272840.2838390469066808Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT238870.2484996083220893Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC236780.24632535378450326Illumina Single End PCR Primer 1 (97% over 35bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC233160.24255942008782325Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT215460.22414587687477439No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGT188720.19632790255178417Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGTG186600.19412243861892184Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT162260.16880121591803995Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG156400.16270498070739214Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT155660.1619351489572421Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG141260.14695463922459218Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATG124430.1294461684745576Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT122070.12699102937948442No Hit
CGTATGCCGTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGG121800.12671014482199722Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG121670.126574904109133Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT108070.11242664491718586No Hit
TCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG101560.10565420614221704Illumina Single End PCR Primer 1 (97% over 35bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTA99360.10336551715528443Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)