FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11094629
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG10900309.824844075453086Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG801010.7219799778793865No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG476660.42963131079011296Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG398570.3592459017782388Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGT278120.2506798559915794Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG168540.15191134376823237Illumina Single End PCR Primer 1 (100% over 32bp)
CGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG149630.13486706044879915Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCAC144470.13021616135158734Illumina Single End PCR Primer 1 (96% over 33bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAGATC142590.1285216477270218Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTG129050.11631754428201249Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAGATC127600.11501060558221461Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTT123650.11145032429655827Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT122320.11025154604088158Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT121580.10958455663546748Illumina Single End PCR Primer 1 (100% over 31bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119020.10727713382754844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)