FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190141
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2217022.7069375240304163Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT306400.3741083334218544Illumina PCR Primer Index 2 (96% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA291720.35618434407905797No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAA267280.32634358797974294TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAT200180.24441581652867755TruSeq Adapter, Index 3 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT185880.2269557996620571TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAA183770.22437953143908024TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATA156990.19168168167068184TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATT145410.17754273094932063TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT141040.1722070474732975TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAA133130.16254909408763538TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAT126990.15505227565679272TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTTA119830.14631005742147785TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGA112490.13734806275007963TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCACA111210.13578520809348704TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCCAA108190.13209784788808887TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAG107030.1306815108555518TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAC98320.12004677330951932TruSeq Adapter, Index 3 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCA95710.11686001498631099TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTGA95080.11609079746001932TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTA92810.11331917240496837TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCA92800.11330696260296375TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATC92210.11258658428469058TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCT91410.1116098001243202TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATG90740.11079174339001002TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAG88960.10861839863318593TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCCAT88250.10775150269085722TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGT88250.10775150269085722TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC88070.10753172625477389RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTTT87450.10677471853048684TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCGAA86280.10534617169594515TruSeq Adapter, Index 3 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTC85490.1043815973375794TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCACT84250.10286758188900534TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)