FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190141
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2212492.7014064837223195Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400110.4885263880072394No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA207990.25395167189429335Illumina Single End PCR Primer 1 (100% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA203060.24793223950601095Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGTAACTGTGT149600.18265863798926052Illumina Single End PCR Primer 1 (96% over 33bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142360.1738187413379086No Hit
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA142300.17374548252588082Illumina Single End PCR Primer 1 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126380.1543074777345103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)