FastQCFastQC Report
Wed 8 May 2024
EGAF00007947348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10462431
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG181311217.32973913997617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT544770.5206916059948209Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAA500210.47810112200501015TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAA495800.4738860404431819TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAT435330.4160887656033287TruSeq Adapter, Index 18 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT432060.4129632969622452TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAA393430.3760407117619222TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATA365560.3494025432521371TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCT353720.33808586168931487TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAC349240.33380387407094964TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACA332610.3179089066393843TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTA324120.3097941577822592TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACT321960.3077296280376903TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATT313490.2996339951967186TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTA302310.2889481421669591TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAA298070.2848955467424349TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCA296370.28327068536939454TruSeq Adapter, Index 18 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290110.27728737231337536No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAC289930.2771153281679946TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAT270700.25873527863648516TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA269560.25764566571574044TruSeq Adapter, Index 18 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAT266440.2546635671958075TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATC261290.24974119303630293TruSeq Adapter, Index 18 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATC242560.23183904390862886TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAT241010.23035755265673916TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTA238500.22795849262948545TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAC229970.21980551174005356TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTT229810.2196525836108262TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCT227450.21739689370472312TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCA219580.20987474134835393TruSeq Adapter, Index 18 (97% over 38bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT214310.20483767109192885Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAC212170.2027922573635133TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAA209400.20014468912626524TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTT208930.19969546274665995TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACC204920.19586270150790003TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT202930.19396065790063513TruSeq Adapter, Index 18 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA202320.19337761940795595TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCA194560.18596060514043056TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGA194380.1857885609950498TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTC189740.1813536452474573TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAG188990.1806367946417042TruSeq Adapter, Index 18 (97% over 38bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC187280.17900237526058715Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGA183610.1754945862964353TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTC183580.1754659122722052TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAG172180.16456978306475809TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGT172170.1645602250566814TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTT171830.1642352527820733TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATG171260.16369044632170093TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGC158260.1512650358219806TruSeq Adapter, Index 18 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT158230.15123636179775046TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC157880.15090183151506567Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC152020.14530083878211478RNA PCR Primer, Index 29 (96% over 29bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT149240.14264371253678998TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTG149070.14248122639948593TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCC144440.13805586865997013TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCC143880.13752062020767447TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGC141590.13533183635810836TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCT137120.13105940674781988TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTC136920.1308682465862857TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAT133350.12745603770290098TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAG130640.12486581751411312TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCC126570.12097570822689296TruSeq Adapter, Index 18 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGA126570.12097570822689296TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGTA125570.12001990741922217TruSeq Adapter, Index 18 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCT120210.11489681509010669TruSeq Adapter, Index 11 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAG116690.11153239624710548TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCG116450.11130300405326449TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGT114720.10964946865599401TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCC114710.1096399106479173TruSeq Adapter, Index 18 (97% over 38bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG114690.10962079463176387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCA113980.10894217605831762TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCT112510.10753714887104153TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCG109380.10454549234303193TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACG107590.10283460889730121TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG104660.10003411253082578Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)