FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10462431
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG170509916.29735001358671Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG472000.4511379812206169No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCAAGCTGTGT331120.31648476343595483Illumina Single End PCR Primer 1 (96% over 33bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA286600.2739325114784509Illumina Single End PCR Primer 1 (100% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA281770.2693159935774009Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG198220.18945883609650566Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT193040.18450778791277095Illumina Single End PCR Primer 1 (100% over 31bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA176330.1685363564165919Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT170370.16283978360287393Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG166890.15951359679217955Illumina Single End PCR Primer 1 (100% over 32bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG144930.13852421105572882Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT122150.11675106865698805No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGTG120320.11500195317895047Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG111220.10630416582914622Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)