FastQCFastQC Report
Thu 9 May 2024
EGAF00007947350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6506162
Total Bases982.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG105910016.278414217168276Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA424840.6529809740366133No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTA399630.6142330916445057TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACA178520.2743860358841357TruSeq Adapter, Index 9 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC152630.23459299046042814TruSeq Adapter, Index 9 (100% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTAC148890.22884459378662872TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTC120250.18482478610277459TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAC113680.17472666681216975TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT113280.17411186502887568TruSeq Adapter, Index 9 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC111290.1710532261569878Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGC108360.16654980309435885TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTA107920.1658735211327354TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCT107230.16481298805655317TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTT102020.15680519482914812TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAT97840.15038051619372528TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT96720.14865907120050192TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACC93100.14309511506169076TruSeq Adapter, Index 9 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCC90800.13956000480774997TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCT89930.1382228109290854TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAA89270.1372083879866502TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGCT87400.1343341896497505TruSeq Adapter, Index 9 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT85410.1312755507778626Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACG84880.13046093841499795TruSeq Adapter, Index 9 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTG81340.1250199426328456TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTGC79100.12157705264639891TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACA78920.1213003918439166TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCA78100.12004004818816377TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCTC77690.11940987636028737TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGT74050.11381518013231148TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACC70110.10775938256686508TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACACT69560.10691403011483575TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCA68280.10494666440829478TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCCT67770.10416279213459485TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTTT67110.10314836919215968TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACG66960.10291781852342441TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)