FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11215200
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG135697612.099436479064126Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCG384140.34251729795277835TruSeq Adapter, Index 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA344410.3070921606391326No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAA250200.22309009201797564TruSeq Adapter, Index 2 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGT178410.1590787502674941TruSeq Adapter, Index 2 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA171110.15256972679934375Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC137730.12280654825593837Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC129180.11518296597474856Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT115110.10263749197517655Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)