FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683083
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG281969919.203725811534266Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGC1197060.8152647505976777TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT829220.5647451560411393TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGT652920.44467500456137177TruSeq Adapter, Index 9 (97% over 39bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATC652340.44427999215151204TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTT652320.4442663710339307TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTA644260.438777060648639TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTG566000.3854776275527422TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA547310.37274869317295284TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCT522720.35600152910665966TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCA496690.338273644574508TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAA442930.30166008051578813TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGA415690.2831081183699636TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC410720.2797232706509934TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGC393620.2680772151189229TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAT392820.2675323704156682TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAT391210.266435870450368TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTC381470.25980238618824125TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG379510.2584675166652671TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGG374120.25479662547708815TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTA366570.24965465359012137TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCG366500.24960697967858655TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAG365790.24912343000444798TruSeq Adapter, Index 9 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG355360.24202001718576405TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTT351710.23953416322716423TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT351690.23952054210958285TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTT344890.23488936213191738TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGT317750.2164055055739997TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTA313120.21325221685391274TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAA309850.21102516412935896TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAT309310.210657393954662TruSeq Adapter, Index 9 (97% over 39bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC306240.20856655240592184Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCA297530.20263455569923564TruSeq Adapter, Index 9 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTA292490.19920203406873066TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTG278390.18959914617386553TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGA275270.18747425183117197TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTG273940.18656844751201093TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTT265250.1806500719229061TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG265210.18062282968774337TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAA263780.1796489197806755TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAC260220.1772243608511918TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTC259400.17666589503035568TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCC259320.1766114105600302TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCT243780.1660278021993065TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC237990.16208448865950018TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGG235130.16013666884536443TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAT234960.1600208893459228TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAC228870.15587325904239593TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCT226650.15436131499086397TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTC226620.15434088331449192TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGC226550.15429320940295713TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAT222320.15141234303449758TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGC221160.1506223182147782TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAG215140.14652236182278613TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGT214790.14628399226511218TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAA211040.1437300327186055TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATG204910.1395551601799159TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGT203610.13866978753712692TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAA202890.1381794273041976TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCA201680.13735534969052482TruSeq Adapter, Index 9 (97% over 39bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC201550.13726681242624592Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGT199840.13610220687303884TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAC193060.13148464801295479TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCA192310.13097385610365345TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTC188650.12848119158626292TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA188200.1281747164406821TruSeq Adapter, Index 9 (97% over 39bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT183070.12468089978106096Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATT181140.12336646193445887TruSeq Adapter, Index 9 (97% over 39bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT177490.120880607975859Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA177250.12071715456488259TruSeq Adapter, Index 7 (97% over 35bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT175100.11925288442488542TruSeq Adapter, Index 9 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG170940.11641969196796068TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAC167190.113865732421454TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAC164800.1122380088704804TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATA164690.11216309272378289TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCC163550.11138668902164484TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTG163170.11112788778759883TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAG162990.11100529772936651TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAG158470.10792692515597711TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGC154470.10520270163970333TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAG154170.10499838487598279TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGC152110.10359540976510179TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGG152030.10354092529477632TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGG149190.10160672659822191TruSeq Adapter, Index 9 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)