FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683083
Sequences flagged as poor quality0
Sequence length151
%GC68

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG266956018.18119532525969Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6476214.410660894581881No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGT1013190.6900390061133619Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTG640390.43614137439664413Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGT536670.36550225861966457Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT482200.3284051448868061Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTA379060.2581610415196863Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGA363990.24789752942212476Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT362040.2465694704579413No Hit
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGATC333760.22730921019788555Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGATC321270.21880282226832062Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG281680.19183982001600072Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGT209740.1428446600758165Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT180480.12291696505427369Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT175820.1197432446578147Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTAT165720.11286458027922337Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG149650.10192001230259339Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)