FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7377900
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG127735317.313232762710257Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA411140.5572588405914962No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGA318020.4310440640290598TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGT262650.3559956085064856TruSeq Adapter, Index 7 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTA174820.23695089388579407TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGC154820.2098429092289134TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGG143570.19459466785941798TruSeq Adapter, Index 7 (96% over 33bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT107380.1455427696227924Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC92220.12499491725287684TruSeq Adapter, Index 23 (97% over 37bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA87960.11922091652096126No Hit
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC81860.11095298120061263Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT74660.10119410672413559Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG73890.10015044931484568Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)