FastQCFastQC Report
Wed 8 May 2024
EGAF00007947369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10001447
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG162301016.227751844308127Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG789740.7896257411552547No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGT229910.2298767368361798Illumina Single End Sequencing Primer (100% over 33bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197570.19754141575713996No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG184850.18482325607484595Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT179050.1790240952134226Illumina Single End PCR Primer 1 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169410.16938548991960864No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT151310.15128810861068404Illumina Single End PCR Primer 1 (100% over 31bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA149490.14946837192658222Illumina Single End PCR Primer 1 (100% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA129220.12920130457122853Illumina Single End PCR Primer 1 (100% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGTAGATC122360.12234229706961403Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG120850.1208325155350021Illumina Single End PCR Primer 1 (100% over 32bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA116270.1162531781651195Illumina Single End PCR Primer 1 (100% over 25bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGTAGATC107320.1073044730427507Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT100930.10091539754197568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)