FastQCFastQC Report
Fri 10 May 2024
EGAF00007947372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5673639
Total Bases856.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG58267210.26981096259385Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA457010.805497142133999No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAA99270.17496707139809214TruSeq Adapter, Index 3 (96% over 32bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACG95810.16886869256221626TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAT88840.15658380802867436TruSeq Adapter, Index 3 (96% over 32bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT72590.12794257794688735Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAG71880.12669117650946773TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAG62760.11061683691895095TruSeq Adapter, Index 3 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)