FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9354662
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG107203211.459868886764696Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA664930.7108006681588281No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTT328620.3512900840244148TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGC167740.17931166299755139TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCT142940.1528008173892333TruSeq Adapter, Index 7 (97% over 35bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTG141940.15173183167922047TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAA111720.1194270835226329TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAC110560.11818706009901801TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCC109770.11734256138810789TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGA108720.11622012639259441TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCA104560.11177314583894105TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAT104390.11159141826823887TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGT103890.11105692541323246TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC99720.10659925500247898Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)