FastQCFastQC Report
Wed 8 May 2024
EGAF00007947380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474603
Total Bases373.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG111533945.071431659947066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512592.0714029684761557No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT153770.621392603177156Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC112370.45409304037859805Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGT102740.4151777072928466TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTT91070.3680186276344125TruSeq Adapter, Index 6 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGA90680.36644261726022315TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTC83980.33936756724209904TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAT76600.3095446016997474TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCT75280.3042104127409528TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTA75010.3031193286357448TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAC69190.27960040459015045TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCT65120.26315332196720037TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACT63610.2570513330825187TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGC62320.2518383756909694TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCC60980.2464233656873446TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCA60090.24282682919239976TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAA58750.23741181918877494TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGA58480.23632073508356696TruSeq Adapter, Index 9 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG58180.23510841941111363Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCT57420.2320372197075652TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTT55100.22266197850725955TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGC54160.21886338940023914TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTG48870.1974862230426456TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGT48520.19607185475811675TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC47800.1931622971442288Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47400.19154587624762437No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACC46980.18984863430618973TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAC46430.18762605557335862TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCT44490.17978641422482716TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGG44280.17893779325410986TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCC44030.1779275301937321TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGGCTGAACTCCAG43080.17408853056429657Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACA42690.17251252019010727TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCA42570.17202759392112593TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGT40540.16382425787085847TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAT40220.16253112115357493TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCA39140.15816678473274298TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTA38840.15695446906028968TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACA37830.1528730062963635TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTC37420.15121617487734396TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCC37110.14996344868247552TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATC37100.14992303816006042TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAGC35270.14252791255809516TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAAC34640.1399820496459432TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG34600.13982040755628275Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG34300.13860809188382944Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGA33870.13687043941997967TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTC33660.13602181844926237TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAG33580.1356985342699415TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACAGATCGGAAGAGCACACGTCTGAACTCCAGT33120.1338396502388464Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAG32800.13254651352156285TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCA32490.13129378732669442TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCC32200.1301218821766562TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTT31740.1282629981455611TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAA31410.12692945090586247TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAC31080.12559590366616383TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGC30710.12410071433680474TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTA30650.12385825120231407TruSeq Adapter, Index 6 (97% over 38bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG30450.12305004075401187Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG30040.12139320933499231Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAA30040.12139320933499231TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGAC29710.12005966209529366TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATT29660.11985760948321812TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCG29300.11840283067627412TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAAT28930.11690764134691504TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCGGAACTCCAG28690.11593778880895239Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGA28510.1152103994054804TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTG28510.1152103994054804TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTG28320.11444259947959329TruSeq Adapter, Index 6 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAG28310.11440218895717817TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGGC26900.10870430529664758TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAG26340.10644131604140139TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATG25910.10470366357755163TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGAT25720.10393586365166453TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCAAG25340.10240026379989033Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG25010.10106671656019167Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)