FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474603
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG104804542.352045964544615Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226140.9138435538953117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199450.8059878695693814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189770.7668704838715543No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG143000.5778704705360819Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT142170.5745163971756279No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT142170.5745163971756279Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG103190.41699618080152656Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT96130.38846635197645846No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT92830.37513087957947194Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTATGGGTGT79930.3230013056639792Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTGGGAAAGAGT49220.1989005913271745Illumina Single End PCR Primer 1 (95% over 23bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG43960.17764465653682632Illumina Single End PCR Primer 1 (96% over 30bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG43270.17485633049018368Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT42360.1711789729504086No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGTG42350.1711385624279935Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT42000.16972419414346462Illumina Single End PCR Primer 1 (96% over 26bp)
GTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT28530.11529122045031062Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGTAGGGAAAGAGT28040.1133111048519702No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGAGGGAAAGAGT27620.11161386291053554No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27170.10979538940185556No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.1079365053707605No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTA25050.10122835864985211Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)