FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676559
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG41790015.613330399217801Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1384505.172686273681992No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140600.5253013290571962No Hit
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92650.3461534006909618No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAA80080.29919011686273306TruSeq Adapter, Index 1 (96% over 32bp)
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73020.27281296620025935No Hit
CTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70660.26399567504396504No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT69230.25865299438570194Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69170.2584288259664741No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.23634823667253363No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAT63160.2359746226404873TruSeq Adapter, Index 1 (96% over 32bp)
ATTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.22289813151886434No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59210.22121686837465568No Hit
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC56380.2106435912677434Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55180.20616022288318697No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAG51290.19162663703658317TruSeq Adapter, Index 1 (96% over 32bp)
CTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51060.1907673247628765No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAC50290.18789049671611946TruSeq Adapter, Index 1 (96% over 32bp)
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39030.1458215567076982No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35370.13214728313480106No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA35080.13106380244186658No Hit
CTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34810.1300550445553414No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA31190.11653021659526279No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.10786237105178702No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGACTC27970.1044998447633697TruSeq Adapter, Index 7 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG27430.10248232899031928Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)