FastQCFastQC Report
Wed 8 May 2024
EGAF00007947383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676559
Total Bases404.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG41116515.361701348634572Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640632.39348357349866No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA576992.15571560350435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190560.7119588994675626No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104090.3888948459570665No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102830.38418730915328225No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT69560.25988592069145494Illumina Single End PCR Primer 1 (100% over 31bp)
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69200.25854091017608805No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61190.2286144262091738No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG58310.21785434208623833Illumina Single End PCR Primer 1 (100% over 32bp)
CTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.19588583700191178No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49730.1857982581366598No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT46260.17283385122465075Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG45430.1697328547586659Illumina Single End PCR Primer 1 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41970.15680580924986148No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39380.1471292058198605No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC39190.1464193391589724No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG33890.12661779546051477Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTATGCGTGT32610.12183553585032125Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT32310.12071469375418215No Hit
CTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31790.11877190078754102No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT30830.11518520607989587No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA30520.11402700258055212No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT30170.11271935346838982Illumina Single End PCR Primer 1 (96% over 26bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA28970.10823598508383338No Hit
TTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.10393942371530014No Hit
CTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27690.10345372547363985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)