FastQCFastQC Report
Thu 2 May 2024
EGAF00007947384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29711250
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG28496959.591299591905424Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1947900.6556102486431907No Hit
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC1177430.396290967226219RNA PCR Primer, Index 29 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTTAGATCGGAAGAGCACACGTCTGAA1140730.3839387437418486Illumina PCR Primer Index 11 (96% over 28bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAA808610.2721561698010013TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT780950.2628465648533805Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATATAGATCGGAAGAGCACACGTCTGAA753470.253597543018217Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAAGATCGGAAGAGCACACGTCTGAA609180.2050334469266692RNA PCR Primer, Index 32 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGATCGGAAGAGCACACGTCTGAAC582910.1961916782363583Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG528720.17795279565821026Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA494530.16644537001977366TruSeq Adapter, Index 4 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCT487100.16394463376667087TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAT466350.1569607471917203TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGCT436870.1470385796625857TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTA436390.14687702469603264TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAA423540.14255206361226808TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTT400170.1346863561782153TruSeq Adapter, Index 4 (96% over 33bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA397360.13374058647818587TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGA385190.1296444949303715TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAT374460.12603306828221633TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGC350610.11800580588161051TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAG349250.11754806680971013TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAC348650.11734612310151879TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATGTAGATCGGAAGAGCACACGTCTGAA344190.11584500820396315RNA PCR Primer, Index 39 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTAA342390.11523917707938912TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAAAGATCGGAAGAGCACACGTCTGAA337400.11355967857293113Illumina PCR Primer Index 2 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATTCAGATCGGAAGAGCACACGTCTGAA330280.11116327990239389RNA PCR Primer, Index 40 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTACT329680.11096133619420254TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAC325900.10968909083259708TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTATA325310.1094905128528756TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTG322570.10856830325213514TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGAA307500.1034961504480626TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGCA307280.10342210442172578TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTATT304600.10252008919180446TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTACA298320.10040641171273508TruSeq Adapter, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)