FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29711250
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG306628410.320279355462999Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2186350.7358660440068997No Hit
AAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG1816600.6114182338339854Illumina PCR Primer Index 4 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA1778820.5987025116748707Illumina Single End PCR Primer 1 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1526640.5138255711220497No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA1278150.43019058437460556Illumina Single End PCR Primer 1 (100% over 26bp)
ATATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG1056480.3555824813833144Illumina PCR Primer Index 2 (96% over 26bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA866960.2917951954226093Illumina Single End PCR Primer 1 (100% over 25bp)
TAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG862260.2902133030417771Illumina PCR Primer Index 4 (96% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC839300.28248559047498845Illumina Single End PCR Primer 1 (96% over 33bp)
GAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG696370.234379233455341Illumina PCR Primer Index 2 (96% over 26bp)
ACATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG627050.2110480037023013Illumina PCR Primer Index 3 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGT592750.1995035550506963Illumina Single End PCR Primer 1 (96% over 33bp)
TTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG476910.16051495645588792Illumina PCR Primer Index 2 (96% over 26bp)
GTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG411270.13842231477975514Illumina PCR Primer Index 2 (100% over 26bp)
CATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA368840.12414152888215743Illumina Single End PCR Primer 1 (100% over 25bp)
AGATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG338010.11376498800959234Illumina Single End Adapter 1 (96% over 26bp)
GATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA322040.10838991964323277Illumina Single End Adapter 1 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)