FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43718877
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG571333113.068338877963404Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10272972.3497790210851024No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1317000.3012428704424407No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1189310.2720358073241451No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC824520.18859587816036535No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG789320.18054443621687719Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACA767800.17562207739233557TruSeq Adapter, Index 13 (97% over 35bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG706910.16169445523497777No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA658720.15067175673336713No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCT649470.14855596588174028TruSeq Adapter, Index 13 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT642640.1469937116637282Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC606840.138805029232567No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC603660.13807765464789956No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCA593970.13586122077197912TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT564780.1291844710466831Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAC561790.12850055594977886TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCA551400.12612400817157313TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAA550730.1259707562936715TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTA530470.12133660249324338TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACT517920.11846598895941449TruSeq Adapter, Index 13 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACACT500050.1143785097682175TruSeq Adapter, Index 13 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC494330.11307015045240068Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAT482850.11044428245492216TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACAAA481300.11008974452843333TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTT480420.10988845847984613TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATC475310.10871962699316362TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATA468270.10710933860446598TruSeq Adapter, Index 13 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACAA463210.10595194382508955TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACGCT460410.10531148821594845TruSeq Adapter, Index 13 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTA460380.1053046261915648TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACCTC452950.10360513148587966TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACAAC438180.10022672814766033TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)