FastQCFastQC Report
Wed 15 May 2024
EGAF00007947390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56476804
Total Bases8.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG831110614.715963743274141Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8065381.4280871842535565No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG1319920.23371010866691397Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACT1280440.22671962811493368TruSeq Adapter, Index 11 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAG1125000.1991968242395586TruSeq Adapter, Index 11 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC947500.16776799197065045Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCA635970.11260729272145073TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCT579940.10268640555510188TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGAC577700.10228978254506044TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)