FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56476804
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG879278315.568839554022922Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6246051.1059496213702178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2458660.4353397901198517No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCT1020120.1806263683051187Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG953630.16885339333295135Illumina Single End PCR Primer 1 (100% over 32bp)
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC946810.1676458179184502No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT867510.15360465510760843No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCTTCGGTGT786420.13924654801642103Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT690310.12222894199183083Illumina Single End PCR Primer 1 (96% over 26bp)
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680850.12055391802978087No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCTTCG656700.11627782620277168Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG597870.10586116027387102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)