FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31528867
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG23084027.321550755376018Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2601710.8251834739256567No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGA824040.26136048593182876TruSeq Adapter, Index 25 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGT660230.2094049240653018TruSeq Adapter, Index 25 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAA535660.16989509962410004TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTA453970.14398551016755534TruSeq Adapter, Index 25 (97% over 40bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG443500.14066474383618036Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAA425630.1349969220270427TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACT415650.1318315688286547TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAT396030.12560870011599212TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAA384920.12208494520275658TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTC381660.12105097211390436TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTT372320.11808860749737693TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAC352070.11166592189944535TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAC350480.11116162214138556TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACA350250.11108867311977941TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAT346590.10992783216726436TruSeq Adapter, Index 25 (97% over 40bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTA327510.10387623507054661TruSeq Adapter, Index 25 (97% over 40bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGC325960.1033846220988531TruSeq Adapter, Index 25 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGTCT316680.10044128766187507TruSeq Adapter, Index 25 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)