FastQCFastQC Report
Fri 10 May 2024
EGAF00007947394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12274381
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG8230316.7052749951301Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4222923.4404341856424367No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTGGA209370.17057479314028137TruSeq Adapter, Index 16 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162150.13210442139607692No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG154070.12552160471473062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)