FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41723321
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG578162313.857053708644143Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT3057980.7329186475832066TruSeq Adapter, Index 3 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA2707530.6489248542799362TruSeq Adapter, Index 3 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1837290.440350853183523No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG1777650.4260566890157185Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAA1509950.3618959286582197TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC1499400.3593673667539552TruSeq Adapter, Index 3 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTG1366440.32750029653679774TruSeq Adapter, Index 1 (97% over 36bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTC1322340.31693066810285786TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG1290250.30923952577983904TruSeq Adapter, Index 3 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTA1256460.30114093746276815TruSeq Adapter, Index 1 (97% over 36bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1116560.2676105288934215No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGC1063190.25481912142132696TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGT1033950.24781105032363077TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCA1001500.24003362531951855TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTT997470.23906773863950093TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA994680.23839904786102714No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCT909620.21801236771157312TruSeq Adapter, Index 1 (97% over 36bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC904060.2166797796369086No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA832650.19956465114557875No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG802210.19226897111090463Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAT792240.1898794201928461TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGT790110.18936891432971023TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTC787440.18872898444493427TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGA787110.18864989198726534TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTA773400.1853639598822922TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAG763590.18301275682249743TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT750090.17977715628149543Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCT745740.17873457388495034TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT743630.17822886150409742TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTT739470.17723181718924053TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAC728420.17458341822790185TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAT727260.17430539625548983TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAA724980.1737589392752317TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAC714750.17130707308749465TruSeq Adapter, Index 1 (97% over 36bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC702160.1682895759903676No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAA692600.16599829145910988TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGC681690.16338344687375198TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA675610.16192622825973035TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGA659240.15800276301112273TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAT652380.15635859858806542TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG652370.15635620184692392TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAT649700.155716271962148TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAC649100.155572467493659TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG637600.1528162151809536Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTA635130.1522242201190073TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAA631160.15127271388583857TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG629730.1509299799026065Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCT624170.149597391827942TruSeq Adapter, Index 1 (97% over 36bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG616750.1478190099009616No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTG615130.14743073783604138TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTC611600.1465846882130979TruSeq Adapter, Index 1 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG609820.14615806828991393TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGG591180.1416905428021897TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGACT589910.14138615667722135TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTT577850.1384956868605929TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG569190.13642010903206866Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC567390.13598869562660174No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTG564190.13522173846132718TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCA545730.13079735431414966TruSeq Adapter, Index 1 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCT539060.1291987279727805TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAC534300.1280578791894346TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAG532230.12756175377314763TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCA530430.1271303403676807TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG530030.1270344707220214TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC529960.127017693534031TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGACA523700.12551733357946268TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTT519070.12440764243095606TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCG518500.12427102818589153TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTA517410.12400978340146988TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCC516860.12387796263868833TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAA508900.12197015669006789TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC493630.11831033296702341RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTC488140.11699452208034926TruSeq Adapter, Index 1 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC486740.11665897832054166Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGG483110.11578896128618334TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAT470420.11274749677764147TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG464750.11138854455042062Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG462120.11075820163021059No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT457730.10970603226909958Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGGC457550.10966289092855289TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAGT456780.10947834186065869TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGATA455770.10923627100536892TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAG452560.10846691709895288TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAC442830.10613488796828996TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCGC441690.1058616594781609TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCGT441010.10569868108054006TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCCT440560.10559082772917333TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCCA440340.1055380994240607TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAGC440140.10549016460123105TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGATG434130.10404972317519978TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCCC432880.10375013053251442TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCC420000.10066312794228437TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)