FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41723321
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG661926015.864652768172505Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10433882.500730946129624No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790990.4292539416984568No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGT1333450.31959344751104546Illumina Single End PCR Primer 1 (96% over 33bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC1320610.31651603188538135No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA1220580.29254143024712725Illumina Single End PCR Primer 1 (100% over 26bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC1201860.28805473083027117No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1053920.2525973423831722No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGTAGATC901020.21595117032989775Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA865670.20747869039475547No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA852090.20422391592462164No Hit
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC849110.20350968706445968No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT842260.2018679193825439Illumina Single End PCR Primer 1 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA803130.19248947129592103Illumina Single End PCR Primer 1 (100% over 25bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG579460.13888156218437164No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTG577400.13838783350922618Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG551080.132079610824843No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT534880.12819689017564062No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG510860.12243991795379855Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT502350.12040029124239654Illumina Single End PCR Primer 1 (100% over 31bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA475080.11386437814957251Illumina Single End PCR Primer 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)