FastQCFastQC Report
Wed 1 May 2024
EGAF00007947406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19404493
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG269168013.871426581462346Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGT770630.3971399819619095TruSeq Adapter, Index 6 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA563530.290412122594494No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAA471400.24293342784065525TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG454970.23446631664120263Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTA448220.23098774082888945TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG388930.20043296158266027TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAT375010.19325936524082335TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTA367770.1895282705917645TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTT361180.18613214990981727TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGC358980.1849983918672856TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAC342690.17660342890690317TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTCT300390.15480435381640736TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTC297700.15341807693712997TruSeq Adapter, Index 3 (96% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTA278180.1433585510324851TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTC267540.13787528486314998TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGA262270.1351594190067218TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTT261510.13476775713748357TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTT260950.13447916418120276TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAG258460.1331959562148828TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGT258310.13311865453016475TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTT254280.1310418159340726TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTG251440.12957823737007712TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC245370.1264500958618192Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAA232290.1197093889544035TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTT231470.11928680641127805TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTG219710.11322635432938134TruSeq Adapter, Index 3 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAC219020.11287076657967822TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAA216500.11157209827641465TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAT216020.11132473288531682TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGCT215270.11093822446172646TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTACT214540.11056202292943186TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAC211580.10903660301766194TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAT205550.10592907529199552TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTCA204100.10518182567305417TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG202280.10424389856514159Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTG198910.10250718738180895TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)