FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19404493
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG276567714.252766099067882Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2251071.1600766894553751No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT551190.2840527706650207Illumina Single End PCR Primer 1 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA396480.2043238130468031No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGTAGATC356160.18354512019458585Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGT345970.17829375907940495Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT302610.1559484187502348No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG258410.13317018898664346Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGAC250510.1290989669248251Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG236060.121652237963651Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGT225790.11635964928328714Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG225450.11618443213125951Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTG221940.11437557270885666Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT216920.11178854299362526Illumina Single End PCR Primer 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)