FastQCFastQC Report
Wed 8 May 2024
EGAF00007947409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350550
Total Bases52.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG11642833.212951076879186Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64081.8279845956354301No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGT26220.7479674796747967Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGT17470.49835972043930965Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG15920.4541434888033091Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT14810.42247896163172155Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTG13640.389102838396805Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC13140.3748395378690629Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG12730.36314363143631434Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG10790.3078020253886749Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAT10460.29838824704036515Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG9990.28498074454428757Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT9180.26187419768934533Illumina Single End PCR Primer 1 (100% over 31bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGA9120.26016260162601623Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT7610.21708743403223507No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGT7510.21423477392668663Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT6470.18456710882898303Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT5990.1708743403223506Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG5460.15575524176294395Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG5430.15489944373127942Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGG4930.14063614320353732Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT4790.13664241905576952No Hit
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATG4540.12951076879189843Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT4530.1292255027813436Illumina Single End PCR Primer 1 (100% over 31bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG4210.12009699044358865Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT3910.11153901012694338Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT3900.11125374411638854No Hit
CGTATGCCGTCTTCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT3530.10069890172585937Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)