FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794627
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG18336710.217554957102507Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCG70270.39155768858932805TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57540.32062372849622794No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCT50560.2817298524985972TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCA49970.2784422612609751TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG42130.23475630312036985Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTA40840.227568179905908TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCC38920.21686957791229042TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTG38690.21558797454847164TruSeq Adapter, Index 7 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACA38670.21547653077770476TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAC37600.20951428904167832TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAA37570.20934712338552802TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTC37520.2090685139586109TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTA36940.20583664460637222TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAC35900.20004156852649604TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGC35750.19920574024574464TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAC34090.18995590727209608TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAA33900.188897191449811TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGC33450.18638970660755688TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTT33410.1861668190660232TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGC32480.18098468372536466TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA32460.1808732399545978TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAT31460.1753010514162553TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAT31310.17446522313550392TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTC28880.16092480498733164TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCC28450.15852876391584436TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAC28350.15797154506201008TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT28310.1577486575204764TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC28010.15607700095897364Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGG27910.1555197821051394TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCA27550.1535137942313361TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTT27090.15095058750369852TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACT26070.14526695519458918TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGT25210.14047487305161463TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCT25130.14002909796854723TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTA23900.13317530606638595TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT23890.13311958418100253TruSeq Adapter, Index 10 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCT23820.13272953098331855TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCT23780.13250664344178484TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACC23240.12949766163107987TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACA22550.12565285153962355TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAT22430.12498418891502246TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCG21720.12102793505279928TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAG21670.12074932562588214TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAA21670.12074932562588214TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCC21400.11924483472052967TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAGC21260.11846472832516171TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGA20370.1135054805260369TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCC20250.11283681790143578TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTT20220.1126696522452855TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAA20130.11216815527683467TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTC20030.11161093642300043TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAT19520.10876912026844575TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATG19470.10849051084152862TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCA19400.10810045764384464TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGT19330.10771040444616069TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA19000.10587158222850764TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATC18820.104868588291606TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATA18570.10347554115702037TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT18490.10302976607395298Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)