FastQCFastQC Report
Thu 9 May 2024
EGAF00007947411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794627
Total Bases270.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG20085011.191740679260926Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164900.9188538899726796No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG75500.42070023464485934Illumina Single End Sequencing Primer (100% over 33bp)
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG63660.35472552235088406Illumina Single End Sequencing Primer (100% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGT53340.2972205366351894Illumina Single End Sequencing Primer (100% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48570.27064119730729563No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAGATC44200.24629073339473886Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG28300.15769293563509298Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCAC27640.15401529119978694Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTG24210.13490268451327211Illumina Single End Sequencing Primer (100% over 33bp)
CGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG23210.1293304959749296Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTT21950.12230953841661804Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT21690.120860769396649Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT19270.10737607313386012Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)