FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17781691
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG272009415.297161557919322Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2645321.4876650370316298No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGT636940.35819990348499475Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGTAGATC464740.261358720045242Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT348880.19620181230232828Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGC301430.16951706111640338Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAC287000.16140197239958787Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG260890.14671832954469854Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG257790.1449749632922988Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTG246400.13856949825525594Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT193400.10876355910132507Illumina Single End PCR Primer 1 (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190570.10717203442574726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)