FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432961
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG430003.000779504815553Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61070.426180475253688No Hit
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT56580.39484675437782324Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAA53450.3730038710055612TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAT39890.2783746382490521TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAA35890.25046041029727956TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATA30650.21389277168045745TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATT26890.18765339740579123TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT26150.1824892652347133TruSeq Adapter, Index 3 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT25910.18081441155760694TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAA24930.17397542570942265TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAT23830.16629901302268518TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTTA22890.15973916945401864TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGA21940.15310954031547266TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCACA21760.1518534000576429TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCCAA21220.14808497928415357TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAG20930.14606119775765009TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAC19110.13336022403959355TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCA18620.1299407311155014TruSeq Adapter, Index 3 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTGA18340.12798673515887732TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATC17660.12324131640707597TruSeq Adapter, Index 3 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCA17480.1219851761492462TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCGAA17310.12079882146129588TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGT16880.11779804195648032TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCA16800.11723975739744487TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTA16790.11716997182756544TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCCAT16720.1166814728384094TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAG16620.1159836171396151TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATG16350.11409940675287046TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTTT16290.11368069333359387TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCT16270.11354112219383501TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCACT15560.10858634673239537TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC15540.1084467755926365RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGC15270.10656256520589186TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAT14630.10209628873360825TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)