FastQCFastQC Report
Wed 8 May 2024
EGAF00007947415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432961
Total Bases216.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG478263.33756466505369Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94280.6579383528232798No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA87080.6076927425100892Illumina Single End PCR Primer 1 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86940.6067157445317772No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGTAACTGTGT34570.24124871507319462Illumina Single End Sequencing Primer (100% over 33bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA26120.182279908525075Illumina Single End PCR Primer 1 (100% over 25bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA16190.11298283763479955Illumina Single End PCR Primer 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)