FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42079989
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG750379617.83221949036156Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT2394420.5690163084405749Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAA2121650.5041945234348801TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAA2070010.49192265710905964TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAT1821940.4329706455008817TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAA1658920.3942301410772707TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATA1537650.3654112171939969TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAC1458660.34663982445432673TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCT1422520.33805141916743375TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACA1406210.3341754675838912TruSeq Adapter, Index 18 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT1406140.33415883259855417TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG1355310.3220794568173485Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACT1354370.3218560727285361TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTA1344150.31942736486932066TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATT1298230.3085148144881882TruSeq Adapter, Index 18 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1292850.3072362970437088No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAA1255410.29833895631484125TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA1231220.29259038066763754TruSeq Adapter, Index 18 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTA1230460.29240977225540626TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAC1219570.28982184382225007TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCA1216620.2891207980116155TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAT1131240.26883086875331647TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAT1098120.26096014426239517TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATC1078900.25639265257412497TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTA1000120.2376711647904661TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAT994910.2364330465960911TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAC956650.22734083889613182TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTT948050.22529711212614623TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCT922340.21918731965447996TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCA921750.219047110492353TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAA874130.20773056761017689TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTT857950.2038855095708319TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAC854820.20314168808361616TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACC851740.20240974872878414TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT845580.20094587001912TruSeq Adapter, Index 18 (97% over 38bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT834840.1983935879831147Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA829140.1970390248913801TruSeq Adapter, Index 18 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATC816720.19408750320728457TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCA805790.19149006906822147TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGA805380.19139263558267566TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAG797270.1894653537100497TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTC784220.18636411715792037TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGA766170.1820746673674273TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTC760650.18076287995227375TruSeq Adapter, Index 18 (97% over 38bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC730290.1735480491689292Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC719010.17086744010318064Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGT709070.16850527218531355TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATG706300.16784700205126005TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAG700220.16640213475340976TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTT690500.16409224821803067TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT672510.15981705698639798TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGC641040.15233844286413667TruSeq Adapter, Index 18 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT634610.15081040063960094TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCC609850.14492636868322376TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTG601800.14301334536945814TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC597180.14191543633721007RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCC583200.1385931921227451TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGC578120.13738596747256754TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCC575780.13682988367701332TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAT561280.1333840652857585TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTC561000.13331752534441016TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCT549600.13060839916094086TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAG536110.1274025998438355TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGA516560.12275668608183334TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGTA514270.12221248441866275TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG504410.11986932791260949Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCT504230.1198265522360284TruSeq Adapter, Index 11 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG503740.11971010733866874Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAG491900.11689641839022344TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCG483780.11496676009112075TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCG477560.11348862282259628TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCA471790.11211742474552452TruSeq Adapter, Index 18 (97% over 38bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG467020.11098386931612553Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGT464280.11033272846150222TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCT460530.1094415685327294TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCC454340.10797056054363513TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACG444120.10554185268441965TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG422770.10046818215660655Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)