FastQCFastQC Report
Fri 3 May 2024
EGAF00007947417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42079989
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG775384218.42643542516135Illumina Single End PCR Primer 1 (100% over 30bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA2885390.6856917191684627Illumina Single End PCR Primer 1 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2811870.6682202317115624No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCAAGCTGTGT1762420.41882615511139987Illumina Single End Sequencing Primer (100% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1144460.2719725045555501No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT820370.1949548988712901Illumina Single End PCR Primer 1 (100% over 31bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA806830.19173721742180114Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT784460.18642115139336182Illumina Single End PCR Primer 1 (96% over 26bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG720530.17122865692764322Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG692160.16448673501316743Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG594910.14137598752699293Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT530290.12601951963438013No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCAAGCTGTGTAGATC526390.12509271330845642Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG446440.10609318362702043Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)