FastQCFastQC Report
Wed 1 May 2024
EGAF00007947420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27597388
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG337688812.23625946049677Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA941110.3410141568470176No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCG823650.2984521578636355TruSeq Adapter, Index 2 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAA652560.23645716036604625TruSeq Adapter, Index 2 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG527590.19117388935503607Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA425030.15401095205096946Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGT402720.14592685365730987TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAC364120.13194002272968733TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAA349030.12647211395513228TruSeq Adapter, Index 2 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC325530.11795681533339315Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC315920.11447460172680111Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAT301300.10917699892468084TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACA290920.10541577340580204TruSeq Adapter, Index 2 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTA278730.10099868871648289TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)