FastQCFastQC Report
Thu 2 May 2024
EGAF00007947425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26090220
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6791652.6031401804967533Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5612682.1512582109311458No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA517670.1984153449070188Illumina Single End PCR Primer 1 (100% over 26bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481850.18468606244025537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444390.1703281919431879No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)