FastQCFastQC Report
Fri 10 May 2024
EGAF00007947426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094690
Total Bases618.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG62471115.256612832717495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244150.5962600343371537No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGA190920.4662624032588548TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG161370.394095767933592Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGT157180.3838630030600607TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGC92650.22626865525839562TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGG82660.20187120392508345TruSeq Adapter, Index 7 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTA80280.19605879810193202TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG56330.13756841177231977Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA53790.131365255977864No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT52830.12902075615003822Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC51200.12503999081737568TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG47740.11659002268792021Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCT45760.11175449179302951TruSeq Adapter, Index 23 (97% over 37bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA43700.10672358591248667No Hit
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT42570.10396391424015006Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCA42220.1031091486779219TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT41720.10188805501759597Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTT41580.1015461487927047TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)