FastQCFastQC Report
Thu 2 May 2024
EGAF00007947430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33876636
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9849952.9075938945059363No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG8541772.5214339463930244Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT458180.13524955665609772Illumina PCR Primer Index 2 (96% over 31bp)
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429900.1269016203379816No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA405840.11979938031627463No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC378220.11164626853740732No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG355840.10503994552469732No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA341300.10074790188730665No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)