FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879241
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG41181614.30293608628107Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139050.48293977475313804No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG101340.35196775816960096Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAA66710.23169300520519123TruSeq Adapter, Index 1 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCT62820.2181825001797349TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA54240.18838298009787996TruSeq Adapter, Index 12 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAT51990.18056842063585507TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACT50430.17515032607551784TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT49980.17358741418311283Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGC47980.1666411391057574TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATA46920.162959613314759TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG44720.155318710729668Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTT44550.15472827734809277TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATT44060.15302643995414067TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCT43990.15278332032643327TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAC41920.14559392562137036TruSeq Adapter, Index 1 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA41310.14347531172277694TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAT40970.1422944449596265TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTT40550.14083572719338186TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG40090.1392380839255901Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAT40040.13906442704865624TruSeq Adapter, Index 1 (97% over 37bp)
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA39590.13750151515625125No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT39510.13722366415315704TruSeq Adapter, Index 12 (96% over 33bp)
GGACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGCCAACCGAGCTTTGA39140.13593860326384627No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAC38960.1353134385068843TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAT38630.13416730311912065TruSeq Adapter, Index 1 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT37830.13138879308817847Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGT37830.13138879308817847TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTA37810.1313193303374049TruSeq Adapter, Index 1 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTA37390.12986061257116024TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAA37120.12892286543571724TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACA37010.1285408203064627TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGCT36770.12770726729718007TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTA36450.12659586328480318TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATT36450.12659586328480318TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATA36020.12510241414317175TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAA35830.124442518010823TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAA35710.12402574150618166TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATC35530.12340057674921968TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA35290.12256702373993703TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTC35110.12194185898297502TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACC34580.12010109608747584TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATG34580.12010109608747584TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG33940.11787828806272209Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACCT33160.11516924078255346TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTC32490.11284223863163938TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACA32470.11277277588086583TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG30840.10711156169282113Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC30200.10488875366806737Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATG30100.1045414399141996TruSeq Adapter, Index 1 (97% over 37bp)
GTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATGCAA30070.10443724578803927No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACT30060.1044025144126525TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAC28970.10061679449549377TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)