FastQCFastQC Report
Wed 1 May 2024
EGAF00007947438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21505820
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG228058410.604496829230413Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1951250.9073125321424619No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG473580.22021015706445976Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAA432170.20095490430032426TruSeq Adapter, Index 3 (96% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAT390080.18138345805926023TruSeq Adapter, Index 3 (96% over 32bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACG317890.14781580056003443TruSeq Adapter, Index 3 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAG283860.13199217700138846TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAG270190.1256357581343097TruSeq Adapter, Index 3 (96% over 32bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT267190.124240786912566Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)