FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7871188
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG360143545.7546560951155Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1699412.15902605807408No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG569920.7240584267584512Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT432210.5491038963876863Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGT326390.41466421587186075TruSeq Adapter, Index 9 (96% over 33bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC321480.4084262756778265Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG250230.3179062677705068Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTT239110.3037787942557083TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG218480.27756928179075385Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTC218330.2773787133530542TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAT204910.2603291904601948TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTA201390.2558571844555104TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCT196330.2494286758237765TruSeq Adapter, Index 6 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGA189890.24124693756520615TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAC181350.2303972411788411TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG179150.22760223742591337Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGA176740.2245404378602061TruSeq Adapter, Index 9 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGC176300.22398143710962054TruSeq Adapter, Index 9 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC175420.22286343560844946Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG172590.21926804441718328Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCT170510.2166254954144152TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACT165710.21052730540802736TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCA156560.1989026307083505TruSeq Adapter, Index 6 (97% over 38bp)
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156470.19878828964573075No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCC156170.1984071527703315TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAA153950.19558673989237713TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGC152440.1936683509528676TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGG146180.18571529481953677TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCT143160.18187851694051774TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTT141710.1800363553760881TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCAAG131190.16667115561208803Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACA126720.16099221616863935TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACC126620.16086517054350627TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTG126550.1607762386059131TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGT124220.15781607554031235TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGGCTGAACTCCAG121880.15484320791219827Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAC119140.15136215778355186TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCC113640.14437464840123246TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCT111370.1414907127107115TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG110700.14063950702231987Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCA109140.13865759527024382TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACAGATCGGAAGAGCACACGTCTGAACTCCAGT109120.13863218614521722Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAT105650.13422370295309932TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGT102850.1306664254493731TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACA101730.12924351444788257TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTA99710.12667719282019435TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCA98660.12534321375629703TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCC95560.12140479937717152TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATC95460.12127775375203845TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAAC93380.11863520474927036TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTC93360.11860979562424376TruSeq Adapter, Index 6 (97% over 38bp)
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92990.11813972681125136No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACAAGTCTGAACTCCAG92890.11801268118611828Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG92330.11730122568537303Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGA90310.1147349040576848TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTC88270.11214317330496996TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAGC88000.11180015011711066TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACCTCTGAACTCCAG87720.11144442236673804Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT87080.11063133036588634Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAG86560.1099706931151943TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCA84870.10782362205044524TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCAAACGTCTGAACTCCAG83860.10654046123660113Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAG83560.1061593243612019TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTATGAACTCCAG83480.10605768786109543Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAA82180.10440609473436539TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCC81180.10313563848303457TruSeq Adapter, Index 6 (97% over 38bp)
ATTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.10297047917036159No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAA80350.10208115979443001TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTT80100.10176354573159731TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGAC79720.10128077235609162TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCGGAACTCCAG79560.10107749935587867Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCG79530.10103938566833875TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAC78920.10026440735502697TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTA78810.10012465716738059TruSeq Adapter, Index 6 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)