FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7871188
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG372318547.30143658111076Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100212.6682249236074655No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG829191.0534496190409885No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT465070.5908510888064166No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT434850.5524579008911996Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG377930.4801435310654504Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG350110.44479943815342743Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT333430.42360822788122965Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG313630.3984531941048797Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT305140.3876670205310812No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTATGGGTGT255700.32485566346528627Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTGGGAAAGAGT174810.22208845729513765Illumina Single End PCR Primer 1 (95% over 23bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG173810.22081800104380686Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT151920.1930077137021756Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT140840.17893105843743026No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134340.17067309280378007No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118370.15038390647002714No Hit
CGTATGCCGTCTTCAGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG107440.13649781964298147Illumina Single End PCR Primer 1 (100% over 30bp)
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100830.12810010382168488No Hit
GTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT97830.12428873506769246Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTA94920.12059170737631981Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGTAGGGAAAGAGT94150.1196134560627951No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGAGGGAAAGAGT93870.11925772831242248No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTCGGGAAAGAG93670.11900363706215632Illumina Single End PCR Primer 1 (96% over 30bp)
ATTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.10941169236460876No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAACGAG85730.10891621442658972Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAC82990.10543516429794333Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)